Both E4bp4 flox/flox and E4bp4-LKO were fed HFLMCD diet for 10 weeks before sacrifice for the following assays: (A-B) body weight and liver weight/body weight ratio; (C) serum ALT, TG, and cholesterol; (D) liver TG and cholesterol; (E) Liver histology by H&E staining; (F) Quantification of lipid metabolic gene expression in the liver. (G) Immuoblotting of AKT-PS473, ATK, lipogenic enzymes, and SREBP-1c in the liver. *p <0.05; **p <0.01, compared with E4bp4flox/flox group. All data are expressed as Mean ± S.D..