Table I.
qPCR primer sequences
| Forward | Reverse | |
|---|---|---|
| 18S | TTGACGGAAGGGCACCACCAG | GCACCACCACCCACGGAATCG |
| Acc1 | GAAGCCACAGTGAAATCTCG | GATGGTTTGGCCTTTCACAT |
| Acox1 | TGCTGCAGACGGCCAGGTTC | GGCCAGACTGCCACCTGCTG |
| Acox2 | CCTTTGCCCAACGACACTGGCA | ACCGGGAGGTACCAAGAACCTCTG |
| Acsl3 | GCAGCTGCGTCAGGGTCC | TAAGACCCGCGGGCTCCG |
| Atg12 | GGCCTCGGAACAGTTGTTTA | CAGCACCGAAATGTCTCTGA |
| Atg2a | CACTCTACGCCACTACAT | ATCCAGCACATCCAAGAA |
| Atg7 | CAGAAGAAGTTGAACGAGTA | CAGAGTCACCATTGTAGTAAT |
| Atgl | TTCACCATCCGCTTGTTGGAG | AGATGGTCACCCAATTTCCTC |
| Bax | GATCAGCTCGGGCACTTTAG | TTGCTGATGGCAACTTCAAC |
| Bcl-2l1 | CGGATTGCAAGTTGGATGGC | TCAGGAACCAGCGGTTGAAG |
| Beclin1 | GGCCAATAAGATGGGTCTGA | CACTGCCTCCAGTGTCTTCA |
| Bim | CGGTCCTCCAGTGGGTATTT | TATGGAAGCCATTGCACTGAGA |
| Cd36 | CCAAGCTATTGCGACATGATT | CCGAACCACAGCGTAGATAGACC |
| Cgi58 | CTTGCTTGGACACAACCTG | GAGGTGACTAACCCTTGATGG |
| Chop | CTGCCTTTCACCTTGGAGAC | CGTTTCCTGGGGATGAGATA |
| Cidea | ACAGAAATGGACACCGGGTAG | TGACATTGAGACAGCCGAGG |
| Col1a1 | GAGGCCTCCCCAGAACATCAC | CGATCTCGTTGGATCCCTGG |
| Col1a2 | AGTCGATGGCTGCTCCAAAA | AGCACCACCAATGTCCAGAG |
| Cpt1a | TCTGCATGTTTGACCCAAAA | TTGCTGGAGATGTGGAAGAA |
| Crbn | TCCTTTGCGGGTAAACAGACA | TCGGTTTTCTGGCTTCTTTACTA |
| Cyp4a10 | GGAGCTCCAATGTCTGAGAAGAGT | TCTCTGGAGTATTCTTCTGAAAAAGGT |
| Cyp4a14 | TCTCTGGCTTTTCTGTACTTTGCTT | CAGAAAGATGAGATGACAGGACACA |
| Dhcr7 | ATTGAGTTCAACCCCCGCAT | AACACGTAGATGGCCTGCAA |
| E4bp4 | ATGGGAAGGCTCTTTCTCCACT | TACCCGAGGTTCCATGTTTC |
| Elovl7 | GCCAAGAGCAATGAGGATGG | GGTCCACGGCATGATCGTAT |
| Fasn | TTGGCCCAGAACTCCTGTAG | CTCGCTTGTCGTCTGCCT |
| Fsp27α | GCCACGCGGTATTGCCAGGA | GGGTCTCCCGGCTGGGCTTA |
| Fsp27β | GTGACCACAGCTTGGGTCGGA | GGGTCTCCCGGCTGGGCTTA |
| Gadd45a | TGGTGACGAACCCACATTCA | CGGGAGATTAATCACGGGCA |
| Grp78 | GGTGCAGCAGGACATCAAGTT | CCCACCTCCAATATCAACTTGA |
| Hmgcr | CACAATAACTTCCCAGGGGT | GGCCTCCATTTAGATCCG |
| Hmgcs | AGAAATCCCTGGCTCGCTTG | AGCTTTAGACCCCTGAAGGC |
| Hrd1 | TTTTCGGCCTGTCAGATGGC | GGCCCAGAGACCTGTGAACG |
| Hsl | ACGCTACACAAAGGCTGCTT | TCGTTGCGTTTGTAGTGCTC |
| Idi1 | GACGTCACCCTTGTGCTAGA | TAGAACAGAGATTCCGGCTG |
| Il-1β | TGCAGCTGGAGAGTGTGGATCCC | TGTGCTCTGCTTGTGAGGTGCTG |
| Il-6 | GACAACTTTGGCATTGTGG | ATGCAGGGATGATGTTCTG |
| Lpl | AGAAGGGAAAGGACTCAGCAG | TCAAACACCCAAACAAGGGTA |
| Lss | ATCCAAGCACTGTTAGAGGCAGGT | TCCAGTGTGCTGAAGGAGAAACCA |
| Magl | GAACAAGTCGGAGGGTTCTGC | GAGGACGTGATAGGCACCTT |
| Mcp1 | ACTGAAGCCAGCTCTCTCTTCCTC | TTCCTTCTTGGGGTCAGCACAGAC |
| Mkrn1 | GCGAGAAAGGAGATCCGACC | 5‘-TTCGTATCTGCAGCGGTCTC |
| Mmp2 | AACGGTCGGGAATACAGCAG | CCACCCATGGTAAACAAGGC |
| Mttp1 | CTCCACAGTGCAGTTCTCACA | AGAGACATATCCCCTGCCTGT |
| Noxa | TGGAGTGCACCGGACATAAC | TCGTCCTTCAAGTCTGCTGG |
| Pcsk9 | CACAATGTAGGTTCCTGGCA | GAGGATGGCCTGGCTGAT |
| Plin2 | GTGGAAAGGACCAAGTCTGTG | GACTCCAGCCGTTCATAGTTG |
| Plin3 | TGTCCAGTGCTTACAACTCGG | CAGGGCACAGGTAGTCACAC |
| Plin4 | TCCTGCTCTGAGGGACCCTT | TCTTGCCTTTGGATTTGGGG |
| Plin5 | TGTCCAGTGCTTACAACTCGG | CAGGGCACAGGTAGTCACAC |
| Pnpla3 | CTCCCTCTCGGCCGTATAAT | AGTCGTGGATGCCCTGGTGT |
| Pparα | CCTTCTACGCTCCCGACCCA | CCATGTCCATAAATCGGCACCA |
| Puma | TACGAGCGGCGGAGACAAG | GTGTAGGCACCTAGTTGGGC |
| Rnf44 | AGAGCACAGCTGCGTCCTG | GGGCTCACAACCCGGCA |
| Scd1 | GCCGAGCCTTGTAAGTTCTG | CCTCCTGCAAGCTCTACACC |
| Sqle | GATGGGCATTGAGACCTTCT | TTTAAAAGAGCCCGACAGGA |
| Srebp-1c | AACGTCACTTCCAGCTAGAC | AACGTCACTTCCAGCTAGAC |
| Srebp2 | CCCTATTCCATTGACTCTGAGC | GAGTCCGGTTCATCCTTGAC |
| Syvn1 | GCGTCTAGGACCTTGTCCTTT | CCAGAGACCTGTGAACGCTAGG |
| Tgfβ | GCTGAACCAAGGAGACGG | ATGTCATGGATGGTGCCC |
| Timp1 | AGGTGGTCTCGTTGATTCGT | GTAAGGCCTGTAGCTGTGCC |
| Tnfα | ACTTCGGGGTGATCGGTCCCC | TGGTTTGCTACGACGTGGGCTAC |
| Ube2o | GCTCTATGGCCAAGAAGGTGA | CCCTATTTCACTCCCGGCTC |
| Ulk1 | ACCATTGTCTACCAGTGT | AGTGTCTTGTTCTTCTCTAA |
| Usp10 | CCAGTGCCTCCCAAACCCCG | GTCCTCCTGCCGGCCCTTTTC |
| Wipi1 | CACAGGATGGAGGAGAAT | GATGGAGGTAAGGAAGGT |