Table 2.
Summary Statistics of CRGs Annotated in Genome Scaffolds and Chemosensory Transcriptomes of Three Species of Apoid Wasps
| Species | Ampulex compressa | Cerceris arenaria | Psenulus fuscipennis |
|---|---|---|---|
| Number of scaffolds with annotated CRGsa | |||
| OR-coding genes | 21 | 50 | NA |
| GR-coding genes | 9 | 7 | NA |
| IR-coding genes | 14 | 17 | NA |
| CSP-coding genes | 4 | 5 | NA |
| OBP-coding genes | 5 | 6 | NA |
| Transcriptome of chemosensory tissues (genome-guided assembly)b | |||
| Assembled paired reads | 54,297,198 | 81,619,921 | |
| Contig count | 94,602,641 | 125,197,722 | |
| Transcript count | 29,018 | 49,242 | |
| Mapped reads (%) | 87.1 | 87.4 | |
| Avg. fragment length (bp) | 349.2 | 337.7 | |
| Std. dev | 88.9 | 91.8 | |
| N50 (contigs) | 1,510 | 4,839 | |
| G + C (%) | 44.37 | 42.52 | |
| Transcriptome of chemosensory tissues (de novo assembly)c | |||
| Assembly size (bp) | 299,500,853 | 261,520,523 | 249,077,981 |
| Assembled paired reads | 10,368,996 | 41,558,667 | 12,431,360 |
| Mapped reads (%) | 95.33 | 89.57 | 92.83 |
| Predicted genes | 74,249 | 136,527 | 112,054 |
| Predicted transcripts | 151,692 | 239,673 | 210,850 |
| N50 | 2,490 | 1,238 | 2,569 |
| G + C (%) | 43.63 | 40.91 | 39.83 |
Note.—NA, genome draft not available.
The number of scaffolds does not per se indicate the spread of CRGs across the genome, as the draft genomes of the two studied species have not been assembled to chromosome level. The genome of A. compressa is more contiguous than that of C. arenaria, indicated by the high number of CRG regions located at scaffold ends in C. arenaria (annotated as incomplete, see supplementary data A2, Supplementary Material online).
Tophat2 was used to map reads onto the draft genome scaffolds, and Cufflinks was used to assemble the mapped reads onto the transcriptome scaffolds.
Statistics inferred with the trinityrnaseq plugin scripts bundled with Trinity.