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. 2021 Mar 31;139:104814. doi: 10.1016/j.jcv.2021.104814

Table 1.

Primers and probe of the Marseille-4 variant-specific qPCR.

Name Sequence (5′-3′) Positions*
Primers:
Pri_IHU_ C4_5_MBF GAGGTTTAGAAGAGCTTTTGGTGA 9,460−9,483
Pri_IHU_ C4_5_MBR CCAGGTAAGAATGAGTAAACTGGTG 9,549−9,573



Probe (6FAM-labelled):
Pro_IHU_ C4_5_MBP CCTTATTTCATTCACTGTACTCTG 9,520−9,543
*

In reference to SARS-CoV-2 genome GenBank Accession no. NC_045512.2 (Wuhan-Hu-1 isolate). The nucleotide carrying the mutation specific to the Marseille-4 variant is covered by the probe and underlined.