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. Author manuscript; available in PMC: 2021 Mar 31.
Published in final edited form as: J Med Chem. 2020 Oct 2;63(20):11819–11830. doi: 10.1021/acs.jmedchem.0c01021

Figure 7. Effects of ZCM-I-1 on mouse brain mitochondria OXPHOS.

Figure 7.

A) Oxygen consumption in isolated mouse brain mitochondria (N=6) was measured using a Clark-type oxygen electrode at 30 °C using pyruvate/malate (20/5 mM) or succinate (20 mM) in the presence of vehicle or ZCM-I-1 (2 μM); B) Detergent solubilized mouse brain mitochondria (N=6) were treated with ZCM-I-1 (2 μM) and the ETC activities were determined; C) 3xTg AD mice (5 months old) were treated with vehicle or ZCM-I-1 (50 mg/kg, oral gavage, 5 times/week, N=8 per group) for 2 weeks. Cortex/hippocampal mitochondria were isolated and oxygen consumption was measured as described above; D) 3xTg AD mice (10 months old) were treated with vehicle or ZCM-I-1 (50 mg/kg by oral gavage, once daily for 8 weeks, N=8 per group). Cortex/hippocampal mitochondria were isolated and measured for oxygen consumption. Data as mean ± SD, statistical analysis by student t-test.