Table 3.

Selective uptake of hHDL₃-derived CE by granulosa cells from WT and Sod2 ± mice in response to FSH or Bt₂cAMP stimulation.

Treatment	WT	Sod2+/−
	Selective HDL-CE uptake (ng/105 cells/24 h) Mean ± SE
FSH (100 ng/ml)	6791 ± 1296	6801 ± 1523
Bt2cAMP (2.5 mM)	8423 ± 1300	7905 ± 1070

Seventy two hours cultured granulosa cells from WT and Sod2+/- mice were treated with FSH (100 ng/ml) or Bt₂cAMP (2.5 mM) for 24 h and then replaced with respective medium containing fresh FSH (100 ng/ml) or Bt₂cAMP (2.5 mM) and [¹²⁵I]DLT-[³H]COE-hHDL₃ (100 μg protein/ml) for an additional 24 h. At the end of incubation, the dishes were processed for the determination ¹²⁵I and ³H radioactivity and calculation of amount of HDL-CE selectively taken up by cells. Results are mean ± SE of four separate determinations.