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. Author manuscript; available in PMC: 2021 Mar 31.
Published in final edited form as: Biochem J. 2021 Mar 26;478(6):1261–1282. doi: 10.1042/BCJ20200984

Figure 2. TEM analysis of IAPP trafficking in rat insulinoma cells.

Figure 2.

Synchronized control (rIAPP) and hIAPP-expressing rat insulinoma (INS 832/13) cells were harvested and processed for TEM analysis as described in material and methods. (A-B) Control rat INS 832/13 cells were examined for rat IAPP intracellular distribution. Representative EM image depicts rat IAPP immuno-localization in the nucleus (arrowhead) of control cells. (C-D) EM analysis revealed secretion and extracellular accumulation of immuno-gold conjugated hIAPP antibodies in hIAPP-expressing cells (depicted by black arrowheads). (E-F) TEM shows nuclear (black arrowheads) and cytosolic (white arrowheads) accumulation of IAPP in hIAPP-expressing cells. (G-H) hIAPP was also found in the mitochondria (white arrowheads) and autophagosomes (black arrowheads). (I, J) Immuno-quantitative analysis of IAPP accumulation in the nucleus and cytosolic compartments of control (rIAPP-expressing, black) and stable hIAPP-expressing (gray) INS 832/13 cells. *p<0.05, Student’s t-test. Magnified areas in original images (white box) are presented on the right. Bar, 500 nm.