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. Author manuscript; available in PMC: 2021 Mar 31.
Published in final edited form as: Biochem J. 2021 Mar 26;478(6):1261–1282. doi: 10.1042/BCJ20200984

Figure 3. Biochemical analysis of IAPP trafficking in human islet cells.

Figure 3.

Human islets were cultured in basal (5mM) and high glucose (20mM) media for 4 days, followed by cell fractionation as described in the method section. (A) Western blot analysis of insulin and hIAPP distribution within the main cellular compartments under the basal and stress (high glucose) conditions. The following organelle-specific marker protein antibodies were used to determine hIAPP and insulin distribution within subcellular fractions: CT, the cytoplasmic fraction (HSP90 and VAMP), OR, ER-enriched organelle fraction (calnexin), and NC, the chromatin-bound fraction (histone). (B-F) The densitometric analysis of hIAPP accumulation in the enriched fractions of cultured human islets. Significance was established at *p< 0.05, **p< 0.01, ANOVA followed by Tukey’s post hoc comparison test. Data represent mean ± SEM of three independent experiments.