Extended Data Figure 6. NrCAM is a novel tripartite synaptic protein.

a, A high magnification STED image showing that endogenous NrCAM was enriched at biotinylated proteins in vivo. b, Immunoblot analysis of endogenous NrCAM, astrocyte marker GFAP, neuronal marker b-Tubulin III or loading control α-Tubulin from mouse brain or purified astrocyte lysate. c, Schematic of the visualization of astrocytic membrane and endogenous NrCAM in vivo. d, STED images demonstrating the localization of endogenous NrCAM in vivo. Coronal sections were immunostained with anti-NrCAM antibody (cyan). High magnification image was shown (right panel). e, Schematic of the visualization of both astrocytic and neuronal NrCAM in vivo. f, STED images demonstrating that the colocalization of astrocytic NrCAM with neuronal NrCAM in vivo. Coronal sections were prepared and co-immunostained with an anti-V5 (cyan) and anti-HA (magenta) antibody. A high-magnification image is shown in the right. n= 3 biological repeats. Data represent means ± s.e.m.