. 2021 Mar 19;17(3):e1009108. doi: 10.1371/journal.pgen.1009108

Table 2. RESCUE OF wupA MUTANTS.

	DL		SDL	RL			V
	18242^DL	13193^DL	24597^SDL	23437^RL	PL87^RL	PG31^RL	hdp²	hdp³
A	-	-	-	-	-	-	-	-
B	-	-	-	-	-	-	-	+^(b)
C	-	-	-	-	-	-	-	-
D	-	-	-	-	-	-	-	-
E	-	-	-	-	-	-	-	+^(b)
F	-	-	-	-	-	-	-	-
G	-	-	-	-	-	-	-	-
H	-	-	-	-	-	-	-	-
I	-	-	-	-	-	-	-	-
J	-	-	-	-	-	-	-	-
K	-	-	-	-	-	-	-	-
Dp1	+	+	+	+	+	+	+	+
Dp2	+ ^(a)	+ ^(a)	+	+	+	+	-	-
Dp3	+	+	+	+	+	+	+	+

Notes.—The wupA transcripts are depicted in white or black (A-K) according to their corresponding ATG site (see Fig 2). Every isoform was driven as UAS constructs by tub-Gal4 and a minimum of 100 offspring adults per cross were counted. Black transcript isoforms K, L and M encode the same protein, thus only K was assayed. (a) Rescue is dose dependent; one copy of Dp2 can rescue ♀DL/+ but two copies are needed to rescue ♂DL or ♀DL/DL. In the last case, only escapers are recovered as adults (<1% viability). The same driver was used to co-overexpress several transcripts (C+H, J+F and J+G) but none of these combinations rescued 18242^DL neither in males nor in heterozygous females. (b) Rescue of hdp³ by either of these two transcripts was obtained for 25% (B) and 20% (E), respectively. The rescue, however, affected to wing position but not to flight. The joint overexpression of C and H also failed to rescue hdp³.

Abbreviations: Dp1 (duplication 1) = Dp(1;2R) CH322-143G12r; Dp2 (duplication 2) = fosmid, wupA-2XTY1-sGFP-V5-preTEV-BLRP-3XFLAG; Dp3 (duplication 3) = Dp(1;3R) JC153