. 2021 Mar 19;17(3):e1009108. doi: 10.1371/journal.pgen.1009108

Table 3. FUNCTIONAL PROPERTIES OF A Dominant Lethal MUTATION IN THE white ATG SITE OF wupA.

Genotype	Rescue/Phenotype
♂ 18320C; Dp3/+	+
♂ 18320C; Dp2/+	+
♂ 18320C; Dp1/+	+
♀ 18320C/+; +/+	Lethal
♀ 18320C/18320C; Dp1/Dp1	+
♀ 18320C/18320C; Dp1/+	Lethal
♀ 18320C/18242^DL ; Dp3/+	<10%
♀ 18320C/18242^DL ; Dp2/+	Lethal
♀ 18320C/18242^DL ; Dp1/+	Lethal
♀ 18320C/23437^RL ; Dp3/+	+
♀ 18320C/hdp³ ; Dp2/Dp2	hdp

Notes.- Assays were carried out with two independent CRISPR/Cas9 mutations (ATG> XYZ) induced on the white ATG site (see Fig 2). Table shows the results for mutant 18320C which were identical to those from 18320B. Mutant 18320C was validated by PCR and sequencing (see Materials and Methods and S5 Fig). A minimum of 100 adult offspring per cross were counted. The data indicate that 18320C and 18320B belong to the DL type of wupA mutants.

Abbreviations: Dp1 (duplication type 1) = Dp(1;2R) CH322-143G12r; Dp2 (duplication type 2) = fosmid, wupA-2XTY1-sGFP-V5-preTEV-BLRP-3XFLAG; Dp3 = Dp(1;3R)JC153