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. 2021 Mar 19;17(3):e1009461. doi: 10.1371/journal.pgen.1009461

Fig 3. MiR397 represses strawberry fruit ripening by targeting LAC11a.

Fig 3

(A) Schematic diagram of LAC11a presenting the target site of miR397. Boxes indicate exons (black) and untranslated regions (white, UTR) separately; black lines indicate introns. Positions of cleaved LAC11a are indicated by arrows, and numbers of 5’ RACE sequenced clones are shown above the arrows. (B) qRT-PCR analysis of LAC11a expression in LAC11a OE strawberry fruits. Agrobacterium tumefaciens-mediated transient transformations were performed on immature Falandi fruits at the big green stage, and strawberry fruits transformed with empty vector were used as control. (C) Phenotypic analysis of LAC11a OE fruits. Scale bar: 1 cm. (D) Total anthocyanin content of fruits from control and LAC11a OE. (E) qRT-PCR analysis of LAC11a expression in FRILAIR OE strawberry fruits. (F) qRT-PCR analysis of LAC11a expression in miR397i strawberry fruits. (G) qRT-PCR analysis of LAC11a on FRILAIR mut1 and FRILAIR mut2 over-expression fruits. (H) Relative expression levels of anthocyanin biosynthesis-related genes in fruits of LAC11a OE and control. All analyses were conducted five days after infection. GAPDH was used as the internal control. Statistically significant differences from control were determined by Student’s t-test: *P <0.05; **P <0.01. Values are means ±SEM of three biological replicates.