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. 2021 Mar 19;17(3):e1009461. doi: 10.1371/journal.pgen.1009461

Fig 4. Transient co-expression of FRILAIR and miR397 in strawberry fruits.

Fig 4

(A) qRT-PCR analysis of mature miR397 expression in strawberry fruits. U6 was used as the internal control. (B) qRT-PCR analysis of FRILAIR expression in strawberry fruits. GAPDH was used as the internal control. (C) qRT-PCR analysis of endogenous LAC11a expression in strawberry fruits. GAPDH was used as the internal control. (D) Phenotypic analyses of strawberry fruits. Agrobacterium tumefaciens-mediated transient transformations were performed on immature Falandi fruits at the big green stage. (E) Measurement on total anthocyanin contents of strawberry fruits. Strawberry fruits were co-transformed with both FRILAIR and miR397 over-expressing vectors. 1–3: strawberry fruits co-expressing OD600 = 0.4 miR397 OE and OD600 = 0.4 FRILAIR OE; 4–6: strawberry fruits coexpressing OD600 = 0.4 miR397 OE and OD600 = 1.0 FRILAIR OE. All analyses were conducted five days after infection, and strawberry fruits transformed with empty vector were used as control. Statistically significant differences from control were determined by Student’s t-test: *P <0.05; **P <0.01. Values are means ±SEM of three biological replicates. Scale bar: 1 cm.