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. 2020 Aug 20;6(10):5493–5506. doi: 10.1021/acsbiomaterials.0c00540

Table 1. Proteomic Analysis of Decellularized Heartsa.

Accession Protein ID Score # unique peptides # peptides # PSMs MW (kDa) Calc.d pI
F1LTJ5 heparan sulfate, Hspg2; OS = Rattus norvegicus GN = Hspg2 PE = 4 SV = 2 [F1LTJ5_RAT]E = 1 528.47 13 20 25 216.3 5.67
P02454 collagen alpha-1(I) chain; OS = Rattus norvegicus GN = Col1a1 PE = 1 SV = 5 [CO1A1_RAT] 372.44 8 8 9 137.9 5.92
F1M566 heparan sulfate, Hspg2 (fragment); OS = Rattus norvegicus GN = Hspg2 PE = 4 SV = 2 [F1M566_RAT] 345.11 3 10 11 230.7 6.95
F1MA59 collagen IV, Col4a1; OS = Rattus norvegicus GN = Col4a1 PE = 4 SV = 1 [F1MA59_RAT] 334.39 6 6 11 160.5 8.29
F1M6Q3 collagen IV, Col4a2; OS = Rattus norvegicus GN = Col4a2 PE = 4 SV = 2 [F1M6Q3_RAT] 165.72 6 6 6 166.1 8.65
F1LS40 collagen alpha-2(I) chain; OS = Rattus norvegicus GN = Col1a2 PE =4 SV = 2 [F1LS40_RAT] 219.09 6 6 8 129.8 9.22
D4A115 collagen VI, Col6a3; OS = Rattus norvegicus GN = Col6a3 PE = 4 SV = 2 [D4A115_RAT] 161.83 2 2 3 240.0 6.38
F1LPD0 collagen XV, Col15a1 (fragment); OS = Rattus norvegicus GN = Col15a1 PE = 1 SV = 2 [F1LPD0_RAT] 149.06 5 5 5 137.0 4.91
F1M0B2 protein LOC683295 (fragment); OS = Rattus norvegicus GN = LOC683295 PE = 4 SV = 2 [F1M0B2_RAT] 144.17 2 2 2 57.7 7.88
P02770 serum albumin; OS = Rattus norvegicus GN = Alb PE = 1 SV =2 [ALBU_RAT] 137.08 5 5 7 68.7 6.48
G3 V763 collagen alpha-1(V) chain; OS = Rattus norvegicus GN = Col5a1 PE = 4 SV = 2 [G3 V763_RAT] 135.61 2 2 3 161.7 4.83
Q6IMF3 keratin, type II cytoskeletal 1; OS = Rattus norvegicus GN = Krt1 PE = 2 SV = 1 [K2C1_RAT] 114.80 3 3 3 64.8 7.87
Q6IFW6 keratin, type I cytoskeletal 10; OS = Rattus norvegicus GN = Krt10 PE = 3 SV = 1[K1C10_RAT] 116.13 5 5 5 56.5 5.15
F1M6Q3 protein Col4a2; OS = Rattus norvegicus GN = Col4a2 PE = 4 SV = 2 [F1M6Q3_RAT] 95.31 3 3 4 166.1 8.65
Q9WVH8 fibulin-5; OS = Rattus norvegicus GN = Fbln5 PE = 2 SV = 1 [FBLN5_RAT] 86.76 2 2 3 50.1 4.67
F1LST1 fibronectin; OS = Rattus norvegicus GN = Fn1 PE = 4 SV =2 [F1LST1_RAT] 85.80 3 3 4 202.4 539
F1LR02 procollagen, type XVIII, alpha 1, isoform CRA_a; OS = Rattus norvegicus GN = Col18a1 PE = 4 SV = 2 [F1LR02_RAT] 69.29 2 2 2 134.6 6.29
F1LNH3 procollagen, type VI, alpha 2, isoform CRA_a; OS = Rattus norvegicus GN = Col6a2 PE =4 SV = 2 [F1LNH3_RAT] 34.71 2 2 2 109.6 6.61
D4A9H2 dermatopontin (predicted), isoform CRA_c; OS = Rattus norvegicus GN = Dpt PE = 4 SV = 1 [D4A9H2_RAT] 54.82 2 2 2 20.0 5.15
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Legend: Protein ID, protein identifier; score: relative abundance of the protein in tested sample; PSM: peptide-spectrum matching; MW, molecular weight; Calc.d pI, calculated isoelectric point.