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. Author manuscript; available in PMC: 2021 Aug 21.
Published in final edited form as: ACS Chem Biol. 2020 Jul 28;15(8):2273–2280. doi: 10.1021/acschembio.0c00481

Figure 3.

Figure 3

Native mass spectrometry of hCp149 and wCp149. Spectra at 20V collision energy (top) and 120V of collision energy (bottom) for human Cp149. A), woodchuck Cp149. B). The +11 dimer peak is indicated by a red box. As collision energy is increased, the intensity of the dimer decreased. Monomer peaks are indicated by one diamond, dimer peaks by two diamonds, and peaks that have contribution from both monomeric and dimeric species are indicated by three diamonds C) Percentage of the +11 dimer peak intensity left at 120V collision energy. Error bars are representative of the standard deviation of three experiments. Percentages of the dimer +11 peak left at other collision energies can be found in Supplemental Material Figure 5. The intensity of the +11 peak at 120V was divided by the intensity of the +11 peak at 20V. The intensity of the peak is proportional to how much of the dimer species is present in the sample. The +11 peak intensity is a measure of the relative stability of the dimers.