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. 2021 Apr 1;131(7):e140625. doi: 10.1172/JCI140625

Figure 2. Motor abnormalities in RIMBP1-KO mice.

Figure 2

(A) Open-field locomotor activity. Left: Locomotor activity as a function of time (bin width, 5 minutes). P value by 1-way ANOVA. Middle: Total activity in RIMBP1-WT and RIMBP1-KO mice. Right: Comparison of relative activity in the periphery (P, left) and the center (C, right) of the open field arena in control and mutant mice. (B) Beam-walk test. Left: Cumulative distribution (left) and summary graphs (right) of total time to cross the beam in RIMBP1-WT and RIMBP1-KO mice. Right: Cumulative distribution (left) and summary graphs (right) of total number of slips in RIMBP1-WT and RIMBP1 KO mice. (C) Limb-clasping test. Left: Representative pictures of a RIMBP1-WT (left) and a RIMBP1-KO (right) mouse during the tail suspension test used to measure limb clasping. Arrows highlight limb clasping in the RIMBP1-KO mouse. Left: Percentage of RIMBP1-WT and RIMBP1-KO mice displaying limb clasping in tail suspension test lasting 10, 20, or 30 seconds. Right: Clasping index in control and mutant mice tail-suspended for 10, 20, or 30 seconds. (D) Abnormal movements and postures triggered by systemic oxotremorine. Top: Examples of abnormal postures (arrows) found in RIMBP1-KO mice after oxotremorine treatment (0.01 mg/kg). Bottom, left: Time course of abnormal motor scores after oxotremorine treatment (0.01 mg/kg). Motor scores were assessed in 2-minute bins. Abnormal motor score scale: 0 = normal motor behavior, 1 = no impairment but slightly slowed movements, 2 = mild impairment with occasional abnormal postures and movements and ambulation with slow walk, 3 = moderate impairment with frequent abnormal postures and movements with limited ambulation, 4 = severe impairment with sustained abnormal postures without any ambulation or upright position. P value by 1-way ANOVA. Bottom, right: Summed abnormal motor scores recorded for 60 minutes after drug injection. Data are presented as mean ± SEM. Number of experiments: (A, right) 19 WT, 16 KO; (B) 10 WT, 10 KO; (C) 10 WT, 10 KO; (D) 9 WT, 10 KO. *P < 0.05; ***P < 0.001 by 1-way ANOVA or Student’s t test (in bar graphs in A–D). NS, not significant.