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. 2021 Apr 1;131(7):e94229. doi: 10.1172/JCI94229

Figure 1. The effects of macrophage SR-BI deficiency on autophagy.

Figure 1

(A–D) WT and Sr-b1–/– macrophages were exposed to different levels of starvation (A), oxidized LDL (B), free cholesterol (FC) enrichment (C), or rapamycin (D). (A) For starvation, the cells were incubated for 12 or 24 hours in DMEM without serum. (B) Cells were incubated for 24 hours in DMEM containing 50 or 100 μg/mL oxidized LDL. (C) Cells were incubated for 24 hours in DMEM containing 50 or 100 μg/mL acetylated LDL and 5 μg/mL Sandoz 58035 (ACAT inhibitor). (D) Cells were incubated for 24 hours with 150 or 300 nM rapamycin. In A–D, also shown are basal levels for cells that were incubated for 24 hours with DMEM containing 10% FBS. The levels of VPS34, Beclin-1, and LC3II were analyzed by Western blotting and dot quantitation. The blots are representative, and the numbers are the mean of 3 experiments, in which the values are normalized to either basal WT (green, regular font) or basal Sr-b1–/– levels (red, italic font).