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. 2021 Apr 1;131(7):e94229. doi: 10.1172/JCI94229

Figure 2. The effects of free cholesterol (FC) enrichment on mRNA levels of autophagy players in WT versus Sr-b1–/– macrophages.

Figure 2

Macrophages were enriched in FC by incubation for 24 hours in DMEM containing 100 μg/mL acetylated LDL and 5 μg/mL Sandoz 58035. The mRNA levels for key autophagy proteins were then measured by real-time PCR in WT and Sr-b1–/– macrophages with or without FC enrichment, including ATG1, ATG5, VPS34, Beclin-1, ATG7, LC3, IRGM-1, Rab5, Rab7, Rab9, and TFEB. The data are expressed as mean ± SEM from 3 independent experiments (n = 3 per group). *P < 0.05, **P < 0.01 by 1-way ANOVA with Bonferroni’s post hoc test.