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. Author manuscript; available in PMC: 2021 Mar 31.
Published in final edited form as: Immunohorizons. 2019 Jun 4;3(6):186–193. doi: 10.4049/immunohorizons.1900016

Figure 3.

Figure 3.

BMDCs from TLR4KO mice gained responsiveness to LPS after the uptake of exosomes from WT cells. (A-B) In TLR4KO BMDCs, treatment with LPS does not stimulate cytokine production. After incubation of exosomes from WT BMDCs and LPS stimulation for 4 hours, real-time PCR showed that TNFα and IL-6 mRNA levels were significantly increased (sal: saline; Exo(wt/S): exosomes purified from the supernatant of wild-type BMDCs pretreated with saline; Exo(wt/LPS): exosomes purified from the supernatant of wild-type BMDCs pretreated with LPS). (C-D) In the supernatant of WT BMDCs, LPS stimulated a high level of TNFα and IL-6 production. In TLR4KO BMDCs, only preincubation with exosomes from WT BMDCs and treatment with LPS could stimulate the production of the pro-inflammatory cytokines TNFα and IL-6, as measured by ELISA. (The results are expressed as the mean ± SEM of triplicate measurements in each group, *p<0.05, **p<0.01, ***p<0.001).