TABLE II.

Primers used in real-time PCR assays

Genes	Orientation	Sequence
IL-6 F	5’–3’	5’-CCGGAGAGGAGACTTCACAG-3’
IL-6 R	5’–3’	5’-tccacgatttcccagagaac-3’
IL-1β F	5’–3’	5’ -ggcaggcagtatcactcatt-3’
IL-1β R	5’–3’	5’-aaggtgctcatgtcctcatc-3’
TNF-α F	5’–3’	5’-gacgtggaactggcagaaga-3’
TNF-α R	5’–3’	5’ -gccacaagcaggaatgagaa-3’
TLR4 F	5’–3’	5’ -atatgcatgatcaacaccacag-3’
TLR4 R	5’–3’	5’ -TTTCCATTGCTGCCCTATAG-3’
GAPDH F	5’–3’	5’-cgaaggtggaagagtgggag-3’
GAPDH R	5’–3’	5’-tgaagcaggcatctgaggg-3’
rpL32A F	5’–3’	5’-gctggaggtgctgctgatgt-3’
rpL32A R	5’–3’	5’ -actctgatggccagctgtgc-3’

F, forward primer R, reverse primer; rpL32A, 60S ribosomal protein L32 A.