Figure 2.

A) Titers of IgG antibodies from MUC1.Tg mice immunized with the Qβ-MUC1-β-Tf conjugate. For determination of anti-MUC1 IgG titers, the ELISA measurements were performed against the corresponding BSA-MUC1-α-Tf, BSA-MUC1-β-Tf, and BSA-MUC1. Each symbol represents one mouse (n = 5 mice for each group). The titer numbers were determined by regression analysis with log10 dilution plotted with optical density and reported as the highest fold of dilution giving the optical absorbance value of 0.1 over those of the pre-immune control sera (OD ~ 0.2). ***p < 0.001. The p values were determined through a two-tailed unpaired Student’s t-test using GraphPad Prism. B) Titers of anti-MUC1-β-Tf IgG subtypes from a representative mouse immunized with the Qβ-MUC1-β-Tf conjugate indicating the generation of all major subtypes of IgG antibodies.