Figure 4.

Analysis of transformation efficiency of unsynchronized and synchronized cell cultures of C. r. CW15. (A) TE was measured at different time points (h, hours after illumination start) after 3 days of cultivation under synchronizing conditions. Each data point shows mean ± SD, n = 3. Transformations by linearized construct pHyg3 (nonhomologous) are depicted in black, and those by linearized construct HCP (homologue to cpftsy) are shown in yellow. A one-sided t test was performed and the statistical confidence level is shown in relation to the previous related data point as one asterisk for p < 0.05, two asterisks for p < 0.01, three asterisks for p < 0.001, while a hash represents insignificant values of p > 0.05. (B) TE data is displayed in comparison with the corresponding cell number (shown as log₂, gray circles) on the third day of growth under synchronizing conditions (compare Figure 2B). Below, the illumination cycle is depicted as bars, as well as the assumed cell cycle phases.