Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 22;1(3):e85. doi: 10.1002/cpz1.85

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Authors.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Sample data for Alternate Protocol 1 followed by Alternate Protocol 2. (A) Protocol workflow: In‐solution digestion of 5e6 RMPI 1788 cells followed by SP2 cleanup of the sample yields sufficient peptide material for 50 injections of 1 μg per injection. This workflow yields >4000 proteins per injection. PSMs, peptide spectrum matches. (B) Dot plot of percentage of peptides identified as fully tryptic with and without missed cleavages or semi‐tryptic. (C) Number of proteins annotated among various cellular components based on Gene Ontology, as annotated by ProteomeDiscoverer 2.4.