Table 1. Secretion parameters for Syt-7 WT and KO.
Estimated parameters for Syt-7 WT and KO when secretion is measured 60 ms or 600 ms after Ca2+-uncaging, which corresponds approximately to the fusion of the RRP, or the RRP + SRP, respectively. Syt-7 elimination resulted in an increase in the reverse priming rate (k-1), and a reduction in forward priming rate (k1*DP, where DP is the size of the Depot Pool, and k1 is the rate constant for priming) following stimulation.
| 60 ms (approx. RRP) | 600 ms (approx. RRP+SRP) | |||
|---|---|---|---|---|
| Syt-7 WT | Syt-7 KO | Syt-7 WT | Syt-7 KO | |
| Pool size (fF) | 74.2 ± 3.3 | 33.6 ± 0.77 | 145 ± 4 | 85.4 ± 2.7 |
| k-1 (s−1) | 0.043 ± 0.0083 | 0.091 ± 0.028 | 0.059 ± 0.0075 | 0.19 ± 0.038 |
| k1 (Before Stim) (fF/s) | 3.21 ± 0.63 | 3.07 ± 0.95 | 8.60 ± 1.12 | 15.8 ± 3.32 |
| k1 (After Stim) (fF/s) | 3.78 ± 0.78 | 1.90 ± 0.62 | 9.8 ± 1.3 | 11.29 ± 2.44 |