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. 2021 Mar 23;2021:5542545. doi: 10.1155/2021/5542545

Figure 1.

Figure 1

Sirt-1 positively promotes cell proliferation in SK-N-SH cells. (a) Stable knockdown of Sirt-1 by introducing shRNA targeting to Sirt-1 mRNA was performed, and knockdown efficacy was confirmed in four individual clones. (b) Stable overexpression of Sirt-1 was measured by semiquantitative western blot analysis to confirm the Sirt-1 protein level. After knockdown (c) or overexpression (d) of Sirt-1, cell viability from days 1-5 and cell cycle distribution were analyzed. ∗P < 0.05, vs. scrambled group. (e) EdU staining was then performed to further confirm the effect of Sirt-1 on the proportion of proliferating cells.