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Sirt-1 failed to affect cell proliferation in SK-N-AS cells. (a) After overexpression or knockdown of Sirt-1, the protein levels of β-actin, Sirt-1, and FOXO were measured by western blot. (b) Cell viability from days 1-5 was measured to access the effect of Sirt-1 on SK-N-AS cells. (c) Cell cycle distribution was then measured by performing PI staining followed by flow cytometry. ∗P < 0.05, vs. vector group.
