Table 1.

Map isolates that generated inconsistent typing results. The Map K10 and Map Telford reference strains are included for comparison

Isolate	Host	Mycobactin Dependency	IS900a PCR	IS1311 PCR Result	IS1311 Sequencing Result	LSP-20b	LSP-18b	LSP-4b	LSP Strain Type Result	Phylogenetic Cluster	No. of SNP’s compared to K10 reference	ANI percentage identity compared to K10 reference	SNP based assay of Leao et al [13]
Map K10c	Bovine	NTe	+	Type C	Type C	+	–	–	Type C	Type C	0	0.999	Type C
Map Telfordd	Ovine	NTf	+	Type S	Type S	–	+	+	Type S	Type S	1533	0.998	Type S
MAP-150	Bovine	+	+	Type B	Type C	+	–	–	Type C	Type C	33	0.999	Type C
MAP-320	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1506	0.998	Type C
MAP-322	Ovine	+	+	Type S	Type C	+	–	–	Type C	Type C	40	0.999	Type C
MAP-323	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1508	0.998	Type C
MAP-328	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1512	0.998	Type C
MAP-339	Ovine	+	+	Type C	Type S	–	+	+	Type S	Type S	1506	0.998	Type S
MAP-340	Bovine	+	+	Type C	Type S	–	+	+	Type S	Type S	1511	0.998	Type S
MAP-350	Ovine	+	+	Type S	Type C	+	–	–	Type C	Type C	33	0.999	Type C
MAP-372	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1505	0.998	Type C
MAP-403	Bovine	+	+	Type S	Type C	+	–	–	Type C	Type C	42	0.999	Type C
MAP-551	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1507	0.998	Type C
MAP-552	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1501	0.998	Type C
MAP-554	Bovine	+	+	Type C	Type S	–	+	+	Type S	Type S	1491	0.998	Type S
MAP-555	Ovine	+	+	Type S	Type C	+	–	–	Type C	Type C	60	0.999	Type C
MAP-556	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1510	0.998	Type C
MAP-557	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1511	0.998	Type C
MAP-571	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1514	0.998	Type C
MAP-588	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1503	0.998	Type C
MAP-598	Ovine	+	+	Type S	Type S	–	+	+	Type S	Type S	1512	0.998	Type C
MAP-612	Caprine	+	+	Type C	Type S	–	+	+	Type S	Type S	1513	0.998	Type S
MAPMRI085	Bovine	NT	+	Type C	Type S	+	+	+	–	Type S	1463	0.998	Type S
SRR1793685	Bovine	NT	+	Type C	Type B	+	–	–	Type C	Type B	290	0.999	Type C
MAPMRI049	Ovine	NT	+	Type S	Type S	–	+	+	Type S	Type S	1442	0.998	Type C
MAPMRI058	Caprine	NT	+	Type S	Type S	–	+	+	Type S	Type S	1474	0.998	Type C
MAPMRI074	Bovine	NT	+	Type S	Type C	+	–	–	Type C	Type C	440	0.999	Type C
MAPMRI083	Cervine	NT	+	Type S	Type S	–	+	+	Type S	Type S	739	0.998	Type C
MAPMRI051	Ovine	NT	+	Type S	Type S	–	+	+	Type S	Type S	1464	0.998	Type C
MAPMRI094	Ovine	NT	+	Type S	Type S	–	+	+	Type S	Type S	88	0.998	Type C

^aIS900 PCR is reported to be specific for identifying Map

^b LSP-20 is present in type C strain and absent in type S, LSP-18 is present in type S strain and absent in type C strain, LSP-4 is present in type S strain and absent in type C

^cMap K10 reference strain (NC_002944.2), IS900 PCR and IS1311 PCR and REA results were obtained from published data [33, 34]

^dMap Telford reference strain (NZ_CPO33688.1), IS900 PCR and IS1311 PCR and REA results obtained from published data [35]. Type strains included for comparative purposes

^e+ = positive result, − = negative result, blank space = result unknown

^fNT = Not tested in this study