Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Anal Bioanal Chem. 2020 Nov 18;413(10):2709–2719. doi: 10.1007/s00216-020-03047-z

Figure 1: Method development tissue (pediatric bicuspid aortic valve) displays aberrant ECM production and post-translational modifications ideal for ECM studies.

Figure 1:

a. Movat’s Pentachrome staining with GAGs in blue, elastin in purple, and collagen in yellow. 20x magnification of the square in a is seen in a’. b. Verhoeff van Gieson staining with collagen in red/pink and elastin in purple/black. 20x magnification of the square in b is seen in b’. c. Alcian blue staining shown GAGs present throughout the valve. d. In silico PTM site count analysis of N- and O-linked glycans (d) and hydroxyproline, hydroxylysine, and disulfide modifications (e). Uniprot reported sites for each protein were obtained from the Uniprot compiled Amino Acid Modification database. Both published confirmed sites and sites identified by similarity and consensus sequences were included in d-e.