Figure 3: Culture in the mPC does not alter the phenotype of human intestinal enteroids (HIEs).

RT-qPCR quantification of markers for proliferative, stem-like cells (A) and terminally differentiated (B) intestinal epithelial cells reveal no statistically significant differences between HIEs cultured in 96-well plates, static mPCs, and dynamic mPCs. Data are compiled from three independent experiments. (C) Alcian blue staining reveals no difference in mucin expression between the three experimental conditions, scale = 50 μm. Two independent, representative samples are shown for each experimental condition. The slightly brighter color of the blue stain in the 96-well plate cultures is likely an artifact of the illumination light focused and reflected by the tissue culture plastic well base and walls when imaging these cultures using a dissecting microscope.