Figure 6: Flow promotes the survival of HIE monolayers infected with both commensal and pathogenic bacteria.
DAPI-stained HIE monolayers infected with RFP-expressing E. coli K12 (red), a commensal bacteria, and GFP-expressing EAEC 042 (green) with and without flow. Bacteria and HIE nuclei appear blue, scale = 25 μm. Both strains of bacteria destroy the HIE monolayer in static culture, but do not in the flow condition (top three panels). Co-infection with commensal bacteria reduces EAEC biofilm formation (comparison of the bottom two panels). Images shown are representative of results from three independent experimental runs.