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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Nat Microbiol. 2021 Jan 28;6(4):455–466. doi: 10.1038/s41564-020-00850-3

Fig. 1. Calcification in brain specimens from ZIKV-infected fetuses.

Fig. 1

(a) CT scan of calcifications (red arrows) in the subcortical white matter in a 9-month-old infant infected with ZIKV during second trimester of pregnancy. (b) Brain tissue sections (10μm) from ZIKV-positive human fetuses derived from stillbirths were stained with Von Kossa (top) and H&E (bottom). (c) Serial brain sections from ZIKV-positive human fetal brain tissue were stained with Von Kossa (calcium; black), anti-PDGFRβ (pericytes), anti-GFAP (astrocytes), or anti-NeuN (neurons). Images were taken at 10x magnification (top panel) and 20x magnification (bottom panel) (d) Von Kossa and RNAScope duplex in-situ hybridization with ZIKV RNA and PDGFRβ mRNA (pericytes) in serially sectioned ZIKV-positive human fetal brain tissues. Brightness and contrast were adjusted in magnified insert. Red arrows indicate PDGFRβ+/ ZIKV+ pericytes. The data are representative images from five ZIKV-positive fetal donors.