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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Nat Microbiol. 2021 Jan 28;6(4):455–466. doi: 10.1038/s41564-020-00850-3

Extended Data Fig. 3. ZIKV infection of 3-weeks-old hSTAT2KI mice resulted in brain calcification.

Extended Data Fig. 3

Three-week-old hSTAT2KI were s.c. infected with ZIKV.(a) At 8 dpi, ZIKV RNA was quantified in the brain (MR766 n=7; PRVABC59 n=5). (b) Osteogenic gene expression in mock- or ZIKV-infected hSTAT2KI brains (PBS n=5; MR766 n=6; PRVABC59 n=6) were normalized to GAPDH and presented as fold changes relative to mock controls. (c) Brain sections were stained with H&E or Alizarin red for calcium deposit. Black dotted line in the magnified insert indicates vasculature and blue dotted line in the magnified insert indicates prominent calcification sites. Data are presented as mean ± SEM, using Mann-Whitney U-test (a) or two-way ANOVA followed by Tukey’s multiple comparisons test (b). *P< 0.05, **P< 0.01 and ***P< 0.001. Exact P values in a compared between ZIKV MR766 and ZIKV PRVABC59 group (P= 0.0051). Data in a-c are representative of three independent experiments.