Fig. 1.

a MSM decreases viability of EC cells in a dose-dependent manner. The viability of cells was evaluated after exposure to MSM for 24 h with AlamarBlue® reagent. The results are expressed as a % of control cells (100% viability for non-treated cells). p < 0.05 was considered statistically significant. **p < 0.01, ***p < 0.001. MSM methylsulfonylmethane. b Pre-treatment with MSM before DOX treatment decreases the viability of EC cells as compared to DOX treatment alone. Cells were pre-treated with MSM or experimental medium for 24 h before DOX treatment for 24 h. The viability was determined with AlamarBlue® reagent and expressed as % of Cnt cells (100% viability for non-treated cells). p < 0.05 was considered statistically significant. *p < 0.05, **p < 0.01, ***p < 0.001 as compared to Cnt. MSM methylsulfonylmethane, DOX doxorubicin, Cnt control