Fig. 2.

Creating SC-islets of defined cell composition. Cell surface markers on beta cells (CD49a) and alpha cells (CD26) were conjugated with antibodies bound to iron and then magnetically purified. Representative examples show staining of human cadaveric islets, SC-islets at the end of the six-step differentiation protocol, and SC-islets enriched with beta (SC-beta^e) and alpha (SC-alpha^e) cells by antibody purification and reassembled into islet-like clusters. Note the SC-islets enriched for beta and alpha cells by antibody purification are nearly, but not completely, pure. Some enriched SC-islets were recombined to form a specific ratio of beta:alpha cells (60% beta cells:40% alpha cells), as shown. Green, C-peptide (beta cells); red, glucagon (alpha cells). All clusters contained cells that did not stain for either C-peptide or glucagon and are other endocrine or non-endocrine cells. Scale bar, 50 μm. This figure is available as part of a downloadable slideset