Fig. 10.

AKBA enhanced the sensitivity of CDDP (AKBA 10 μg/ml, CDDP 2 μg/ml) through apoptosis induction via p21-dependent signaling pathway in A549 cells. a Flow cytometry showed the apoptotic cell percentages after treatment of AKBA, CDDP alone, or in combination, in NC siRNA and p21 siRNA groups. b Histogram showing the apoptotic cell percentages of A549 cells and relative statistical analysis. c, d Bax and p21 protein expressions were determined by western blotting assay after knockdown of p21 in A549 cells treated with AKBA, CDDP alone, or in combination and relative statistical analysis. e, f Morphological alterations of the apoptotic nuclei of A549 and relative statistical analysis in each group. Scale bar = 50 μM. Data are represented as the mean ± SD of 3 independent experiments. ^#P < 0.05, ^##P < 0.01, ^####P < 0.0001