Fig. 6.

Effects of cotreatment with AKBA and CDDP (AKBA 10 μg/ml, CDDP 2 μg/ml) on the mRNA and protein expression levels of p27 and p21. a The mRNA expression level of p27 was measured by PCR in each group. b The mRNA expression level of p21 was measured by PCR in each group. c The protein expression levels of p27 and p21 were measured by western blotting assay in each group. d, e Histogram showing the levels of p27 and p21 protein expression and relative statistical analysis. Data were represented as the mean ± SD of 3 independent experiments, *P < 0.05, **P < 0.01, *** P < 0.001, ****P < 0.0001, vs. control group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001