Fig. 1.

A Phylogenetic trees of the BG gene family. Genes from watermelon are labeled with black diamonds. The phylogenetic trees are shown only in the form of topology structures. Sequence data from other species can be found in the GenBank data libraries under the following accession numbers: tomato-SlBG2 (Solyc07 g063880), grape-VvBG1 (XM_002274626), Arabidopsis-AtBG1 (841670) and Arabidopsis-AtBG2 (824893), and strawberry-FaBG3 (JX244263). B Expression of ClBG1 (Cla97C08G153160) in the 97103 and PI296341-FR lines during watermelon fruit development. C Schematic diagram of CRISPR/Cas9-targeted sites in the ClBG1 gene. Orange boxes represent exons; lines represent introns. PAMs (NGGs) are shown in red, and the sequence of each gRNA is shown. D Mutation sites in the Clbg1 homozygous mutant lines from the T4 generation. Frameshift mutations for encoding sequences are also shown