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. 2021 Mar 31;11:7281. doi: 10.1038/s41598-021-86268-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Localisation of DAMPs in term placenta from uncomplicated pregnancies and placental explants cultured under control conditions or exposed to hypoxia (1% O₂), oxidative stress (1 mM H₂O₂) or LPS (1 ng/ml) treatment for 72 h. Representative images from three independent experiments are shown. Immunohistochemistry for: (A–D) high mobility group box 1(HMGB1), (E–H) S100 calcium binding protein A8 (S100A8), (I–L) S100 calcium binding protein A12 (S100A12) and (M–P) heat shock protein 70 (HSP70). Black arrow heads indicate representative positive stromal cells, black arrows show representative positive trophoblast cells, v = vessels. Magnification × 200. Scale bar marked on negative controls represents 50 μm.