Figure 4.

Peritoneal cells from renin KO mice have decreased ability to inhibit bacterial growth in vitro. (a) Peritoneal B-1 lymphocytes were obtained from wildtype and renin-KO mice. These cells were co-cultured with Salmonella typhimurium bacteria for 2 h. A third group included Salmonella typhimurium bacteria alone. Cells were removed after 2 h, and the bacteria were grown in agar for 24 h. Bacteria numbers were then calculated. (b) These experiments were repeated at cell:bacteria ratios of 1:1, 1:2, and 1:5. Each experiment was performed 5 times, and the results were grouped together. Statistical significance was determined using the Mann–Whitney test as values represented paired observations (WT vs KO 1:2 cell:bacteria ratio, P = 0.0362). (c) Schematic as to how the endocrine renin-angiotensin system and the innate immune system coordinate efforts in response to threats to homeostasis.