Fig. 1.

Liver-specific functional analysis of iBHs in PHH/NHDF cell sheets and normal hepatocytes in PHH/3T3-J2 cell sheets. A Albumin secretion on day 4 and day 10. B Urea synthesis on day 4 and day 10. C CYP1A2 activities on day 11. D CYP3A4 activities on day 11. E TGF-β1 secretion on day 4. Measurements of albumin, urea and TGF-β1 were performed in three independent experiments using one lot of PHHs (Hu8200_A). Measurements of CYP activities were performed in three independent experiments using two lots of PHHs (Hu8200_A × 2 + Hu1652 × 1). In each experiment, more than three replicate samples were measured. * *p < 0.01, * p < 0.05, N.S. no significant difference