FIGURE 5.

TLR4 over-expression or SREBP1a over-expression restores liver injury in BRG1 deficient mice. (A–F) WT and BRG1 LKO mice were injected via tail veinadenovirus carrying either TLR4 (Ad-GFP-TLR4), SREBP1a (Ad-GFP-SREBP1a), or GFP (Ad-GFP). 2 week later, the mice were injected with injected peritoneally with LPS (15 mg/kg) and sacrificed 24 h after the injection. Gene expression levels were examined by Western (A). Plasma ALT levels (B). Plasma AST levels (C). Paraffin sections were stained with F4/80 (D). Paraffin sections were stained with TUNEL (E). Gene expression levels were examined by qPCR (F). N = 6 mice for all groups. (G) Primary hepatocytes were isolated from WT or BRG1 LKO mice and transduced with indicated andenoviral particles. The cells were with LPS (1 μg/ml) for 12 h and gene expression was examined by qPCR.