Figure 1.

Antigen-specific immunoglobulin (IgG) is significantly decreased in immunized low dose clodronate treated mice and CD169 knockout mice (A) Total anti-ovalbumin (OVA)-IgG (B) anti-OVA-IgG1 and (C) anti-OVA-IgG2c levels were measure by enzyme-linked immunosorbent assay (ELISA) following immunizations with PBS, OVA alone, or OVA + PorB, OVA + CpG, or OVA + Alum. Wildtype (open bars), wildtype treated low dose clodronate (striped bars), or CD169 knockout mice (checkered bars) were immunized three times at 2-week intervals. The results shown are from sera collected 2 weeks after third immunizations. n = 7–14 per group. Statistics were calculated by ordinary one-way ANOVA with Sidak’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 ****p < 0.0001.