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. 2021 Mar 18;12:624197. doi: 10.3389/fimmu.2021.624197

Figure 3.

Figure 3

Toll-like receptor (TLR) ligand-adjuvant induced increases in the follicular dendritic cell (FDC) network are affected in low dose clodronate treated mice but not CD169 knockout mice. (A) Representative images for FDC networks in draining lymph nodes 24 h post adjuvant+OVA-A594 subcutaneous injections in mice treated with low dose clodronate 24 h prior to these immunizations or CD169 knockout mice. FDC expression is shown as a heat map where white designates the highest signal to pixel ratio and blue designates the lowest signal to pixel ratio. Scale bar is 100 µM. One out of two representative experiments are shown. (B) Mean fluorescence intensity (MFI) quantification from ImageJ of FDC in draining lymph nodes 24 h post injection of either OVA-A594, OVA-A594+PorB, OVA-A594+CpG, or OVA-A594+Alum in all three animal models. Wild type control injections are shown in the bars with no pattern. Low dose clodronate treated animals are shown in the stripped bars. CD169 knockout animals are shown in checkered bars. Multiple FDC networks were measured within the lymph nodes. n = 7–15/group. (C) Frequency of FDC cells in draining lymph nodes 24 h post subcutaneous injections with OVA-A594 +/- adjuvants. (n = 6). Gating strategy is shown in Supplemental Figure 5 . Statistics were calculated by ordinary one-way ANOVA with Sidak’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001.