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. 2021 Mar 18;12:624197. doi: 10.3389/fimmu.2021.624197

Figure 5.

Figure 5

PorB-induced antigen association with dendritic cells (DCs) and follicular dendritic cells (FDCs) is diminished in low dose clodronate treated mice and CD169 knockout mice. These graphs display ovalbumin (OVA)-A594 association with DC and FDC and unassociated OVA within draining lymph node 24 h post subcutaneous immunizations of indicated adjuvants with OVA in WT mice, low dose clodronate mice of CD169 knockout mice. Injection of OVA without adjuvants was used as a control. MFI of OVA=594 associated with DC (A), FDC (B), or unassociated OVA (C) was assessed using the JaCoP plugin within ImageJ and determining the Mander’s correlation coefficient after subtracting the background and using an unsharp mask filter. The correlation coefficient is the percentage of OVA associated with either FDCs or DCs. This percentage was then multiplied by the total MFI of OVA within the lymph node ( Figure 2 ) to determine MFI of OVA associated with DCs or FDCs. Wild type control injections are shown in the bars with no pattern. Low dose clodronate treated animals are shown in the striped bars. CD169 knockout animals are shown in checkered bars. Statistics were calculated by ordinary one-way ANOVA with Sidak’s multiple comparisons test. n = 5–7 *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.