Figure 5.

The omentum regulates the functional activity of peritoneal neutrophils during CLP -induced peritonitis. At indicated intervals, peritoneal cells were collected from WT and omentectomized mice (n = 6 per group). Cells were stained for neutrophil markers (Ly6G⁺CD11b⁺) and assessed for phagocytic capacity (A) or ROS production (B) as described in Materials and Methods. Cells were stained for neutrophil surface markers (Ly6G⁺CD11b⁺) and intracellular cytokine antibodies for TNF-α or IL-6 (C). The bar graph represents the average mean fluorescence intensity (MFI) of TNF-α and IL-6 in neutrophils. Data are representative of three independent experiments with similar results, and data in the bar graph are shown as means ± SEM. **p < 0.01 and *p < 0.05 for omentectomized vs WT.