FIGURE 2.

Fenretininde increases intestinal expression of tight junction proteins. Ileum mRNA levels of tight junction proteins (A) Claudin 1, (B), Occludin, (C) Z O -1, (D) Fordrin, (E) Symplekin, (F) Cingulin were measured using qPCR as described in materials and methods. (G–J) Representative images of hematoxylin and eosin stain ileum sections. Magnification: ×100; Scale Bar = 50 μm. Representative immunofluorescence (IF) images of ileum sections stained with antibodies against: occludin K-N), or claudin one O-R). Magnification: ×100; Scale Bar = 50 μm. Quantification of ileum IF optical intensity of occludin S), and claudin 1 T). All data errors bars represent ±SD, with **p < 0.01, ns = not significant.