Figure 1.

Immunohistochemical confirmation of cMC. Cell nuclei were counterstained blue with DAPI (4′,6-Diamidin-2-phenylindol), MC green with Fluorescein Avidin D (FITC-Avidin) and red with c-Kit staining as described previously (6, 68). Note the large cell nuclei and the intact cytoplasm packed with granules in the double reactive cMC and the small nucleus and narrow rim of cytoplasm surrounding the unstained cell, who's morphology suggests it to be a lymphocyte. The majority of c-Kit negative cells showed this morphology prior to cell culture and were lost in passaging.