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. 2021 Mar 18;12:631881. doi: 10.3389/fimmu.2021.631881

Figure 4.

Figure 4

MC cytokine expression is altered in the presence of SLURP-1. (A–D) immunohistology and (E–H) HM of TNFα, IL1β, IL10, and TGFβ in full-thickness skin samples obtained from 24 h noise-stressed mice. sMC are labeled green, cytokines red. Each dot in the graphs represents one mouse. (I–N) N = 3 WT cMC stimulation and co-stimulation with SLURP-1, AR-R 17779 and IL1β and Chrna7-KO stimulation with SLURP-1. Of note, these experiments were done with cMC stimulated for 2, 8, 24 as well as 32 h and the clearest results reached after 24 h are shown: SLURP-1 24 h, AR-R 17779 8 h pre-incubation prior to 24 h SLURP-1, IL1β 24 h, and SLURP-1 8 h pre-incubation prior to 24 h IL1β. P < 0.01 = *. Scale bar: (A–D) 10 μm. Chrna, alpha nicotinic acetylcholine receptor; IL, interleukin; SLURP, Secreted Ly-6/uPAR-related protein; TGF, transforming growth factor; TNF, tumor necrosis factor.