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. 2021 Mar 18;12:631881. doi: 10.3389/fimmu.2021.631881

Figure 5.

Figure 5

ROS mediated HIF1α upregulation inhibits TNFα production. (A) qPCR of HIF1α and STAT3, (B) immunohistology, and (C) HM of HIF1α in full-thickness skin samples obtained from control and 24 h noise-stressed mice. sMC are labeled green, HIF1α red. (D,E) N = 3 WT cMC stimulation with AR-R 17779 increased HIF1α mRNA and protein while treatment with SLURP-1 or Chrna7 KO did not change levels compared with control. (F) absolute ROS levels measured after 1, 3, and 24 h. wt cMC were stimulated with SP and AR-R 17779. Each dot in (A,C,F) represents one mouse. (G) N = 3 WT cMC co-stimulation with SLURP-1 and a HIF1α inhibitor. Results for AR-R 17779 24 h and HIF1α inhibitor 8 h pre-incubation prior to 24 h SLURP-1 are shown. P < 0.001 = **. Scale bar: (B) 10 μm. HIF, hypoxia inducible factor; ROS, reactive oxygen species; STAT, signal transducer and activator of transcription; SLURP, Secreted Ly-6/uPAR-related protein.