Table 2.
List of primary and secondary antibodies used for immunofluorescence.
| Target | Source | Dilution | Type/Reference |
|---|---|---|---|
| Rabbit polyclonal anti TNFα | Abcam (Cambridge, UK) | 1:200 | ab34674 (60) |
| Rabbit polyclonal anti IL1β | Abcam (Cambridge, UK) | 1:100 | ab9722 (61) |
| Rabbit polyclonal anti IL10 | Abbiotec (San Diego, CA, USA) | 1:100 | 250713 (62) |
| Rabbit polyclonal anti TGFβ | Santa Cruz (Dallas, TX, USA) | 1:250 | sc-90 (63) |
| Goat polyclonal anti ChAT | AbD Serotec (Kidlington, UK) | 1:200 | 2080-0000 (64) |
| Rabbit antiserum SLURP-1 | Yasuhiro Moriwaki (Tokyo, JPN) | 1:200 | (44) |
| Goat polyclonal anti Chrna7 | Abcam (Cambridge, UK) | 1:100 | ab110851 (65) |
| Rabbit polyclonal anti HIF1α | Novus biologicals (Centennial, CO, USA) | 1:400 | NB100-479 (66) |
| Cy3-conjugated F(ab')2 fragment donkey anti-rabbit IgG, polyclonal | Dianova (Hamburg, Germany) | 1:200 | 711-166-152 (67) |
| Cy3-conjugated F(ab')2 fragment donkey anti-goat IgG, polyclonal | Dianova (Hamburg, Germany) | 1:200 | 705-166-147 (67) |
| FITC-Avidin | Vector laboratories (Burlingame, CA, USA) | 1:5000 | (68) |